Principal Investigator: Dr. Farhad Pourfarzi

Professor of Community Medicine, Ardabil Digestive Disease Research Center, Ardabil University of Medical Sciences, Ardabil, Iran.

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 Approval date: 17 March 2016

 Starting date: 13 May 2017

 Goals: Main objectives A) To determine the incidence of major NCDs in people aged 35-69 years B) To identify and measure the association between risk factors and GC C) To determine the etiology of GI cancers D) To stablish a Biobank for collected samples

 Study population: All eligible people aged between ages of 35 and 69 living in Ardabil County

 Sampling method and sample size: Convenient sampling method was used to recruit 10,000 resident of Ardabil city

 Data Collection: All data were collected based on the Persian cohort protocol. People were interviewed in center to collect data about all risk factors of non-communicable diseases including Socio-economic status and lifestyle, Environmental and occupational exposures, General health and medical and family history, Anthropometric measurements, Reproductive history, Dietary habits. All the above-mentioned exposures collected using an internet-based questionnaire. Meanwhile blood, urine, hair and nail samples have been collected and kept in ultra-freezer.

 Follow up Methods: Participants will be actively followed every 4 months. Additionally, a full assessment (as in the enrollment phase) will take place in two stages in a period of 5 years. In active follow up, each participant will be contacted by phone. Annual reports from diseases and deaths records will also be reviewed.

 Main Exposures: all risk factors of non-communicable diseases including: a. Socio-economic status and lifestyle b. Environmental and occupational exposures c. General health and medical and family history d. Anthropometric measurements e. Reproductive history f. Dietary habits

 Outcomes: cardiovascular diseases, cerebrovascular accidents, cancers, diabetes mellitus, chronic pulmonary diseases, chronic kidney disease leading to dialysis, cirrhosis, Parkinson s and Alzheimer s diseases, hospital admission and deaths. In addition, Helicobacter pylori infection has been diagnosed using UBT and HP stool antigen.

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